Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Steven P. Hanlon; Andrea Camattari; Sandra Abad; Anton Glieder; Beat Wirz; Matthias Kittelmann; Stephan Lütz; Margit Winkler

doi:10.1039/c2cc17878h

Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Steven P. Hanlon, Andrea Camattari, Sandra Abad, Anton Glieder, Beat Wirz, Matthias Kittelmann, Stephan Lütz, Margit Winkler

Research output: Contribution to journal › Article › peer-review

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

Original language	English
Pages (from-to)	6001-6003
Number of pages	3
Journal	Chemical Communications
Volume	48
DOIs	https://doi.org/10.1039/c2cc17878h
Publication status	Published - 2012

Keywords

Enzymes
Escherichia coli
Flavins
Humans
Mixed Function Oxygenases
Moclobemide
Journal Article
Research Support, Non-U.S. Gov't

Fields of Expertise

Human- & Biotechnology

Treatment code (Nähere Zuordnung)

Experimental
Application

Access to Document

10.1039/c2cc17878h

Cite this

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title = "Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale",

abstract = "A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.",

keywords = "Enzymes, Escherichia coli, Flavins, Humans, Mixed Function Oxygenases, Moclobemide, Journal Article, Research Support, Non-U.S. Gov't",

author = "Hanlon, \{Steven P.\} and Andrea Camattari and Sandra Abad and Anton Glieder and Beat Wirz and Matthias Kittelmann and Stephan L{\"u}tz and Margit Winkler",

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doi = "10.1039/c2cc17878h",

language = "English",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

AU - Hanlon, Steven P.

AU - Camattari, Andrea

AU - Abad, Sandra

AU - Glieder, Anton

AU - Wirz, Beat

AU - Kittelmann, Matthias

AU - Lütz, Stephan

AU - Winkler, Margit

PY - 2012

Y1 - 2012

N2 - A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

AB - A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

KW - Enzymes

KW - Escherichia coli

KW - Flavins

KW - Humans

KW - Mixed Function Oxygenases

KW - Moclobemide

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1039/c2cc17878h

DO - 10.1039/c2cc17878h

M3 - Article

C2 - 22576266

SN - 1364-548X

VL - 48

SP - 6001

EP - 6003

JO - Chemical Communications

JF - Chemical Communications

ER -