Magnetically Responsive Enzyme and Hydrogen-Bonded Organic Framework Biocomposites for Biosensing

Francesco Carraro*, Margherita Aghito, Simone Dal Zilio, Heimo Wolinski, Christian J. Doonan, Bernd Nidetzky, Paolo Falcaro*

*Korrespondierende/r Autor/-in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftArtikelBegutachtung

Abstract

The one-pot synthesis of multicomponent hydrogen-bonded organic framework (HOF) biocomposites is reported. The co-immoblization of enzymes and magnetic nanoparticles (MNPs) into the HOF crystals yielded biocatalysts (MNPs-enzyme@BioHOF-1) with dynamic localization properties. Using a permanent magnet, it is possible to separate the MNPs-enzyme@BioHOF-1 particles from a solution. Catalase (CAT) and glucose oxidase (GOx) show increased retention of their activity when coimmobilized with MNPs. MNPs-GOx@BioHOF-1 biocomposites are used to prepare a proof-of-concept glucose microfluidic biosensor, where a magnet allow to position and keep in place the biocomposite inside a microfluidic chip. The magnetic response of these biocatalysts can pave the way for new applications for the emerging HOF biocomposites.

Originalspracheenglisch
Aufsatznummer2407487
FachzeitschriftSmall
Jahrgang20
Ausgabenummer52
DOIs
PublikationsstatusVeröffentlicht - 27 Dez. 2024

ASJC Scopus subject areas

  • Biotechnology
  • Allgemeine Chemie
  • Biomaterialien
  • Allgemeine Materialwissenschaften
  • Ingenieurwesen (sonstige)

Fields of Expertise

  • Advanced Materials Science

Fingerprint

Untersuchen Sie die Forschungsthemen von „Magnetically Responsive Enzyme and Hydrogen-Bonded Organic Framework Biocomposites for Biosensing“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren